Biotechnology

Wake Tech offers a variety of non-degree, short classes and certificate training programs in biotechnology, including hands-on training in biomanufacturing and biopharmaceutical operations in a simulated industrial, current good manufacturing practices (cGMP) environment.

For those interested in earning a degree to work in the biotech industry, visit Wake Tech's Biotechnology Department.

Financial assistance available

Workforce Continuing Education offers financial assistance for many workforce training programs through the Propel program and other resources. Email [email protected] for more information.

Course details and registration information

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Can't find what you are looking for? Contact us | View Full Non-degree Catalog | View Available Non-degree Classes

Biowork: Process Technician TrainingBTC-3200A73 available classes

The BioWork Process Technician course is designed to provide basic preparation for entry-level process technician jobs in bioprocess manufacturing, pharmaceutical manufacturing, and chemical manufacturing. BioWork provides competencies to include understanding the role of a...

Aseptic Processing ConceptsSEF-3001JV1

Aseptic processing is utilized to prevent contamination and manufacture sterile products to ensure patient safety. Contamination of these drug products via the introduction of microorganisms, endotoxins, or particulates will lead to product failure, product destruction, and...

Aseptic Processing Level ISEF-79964

Through lecture and hands-on activities, practice the basics of aseptic operations in a simulated CGMP environment. Topics include microbiology, cleanroom design and control, environmental monitoring, cleaning and disinfection, aseptic gowning, component preparation, solutio...

Aseptic Processing Level IISEF-79974

Delve more deeply into the aseptic processing arena with intensive hands-on instruction of aseptic techniques in a simulated cGMP environment. Instruction includes process simulations of closed-system filtrations, hand filling, semi-automated filling, LAF cabinets, BSC's and...

Biomanufacturing Boot CampSEF-79914

This course allows participants to experience biopharmaceutical manufacturing through lectures and hands-on production of green fluorescent protein (GFP) within a current good manufacturing practices (cGMP) environment. The course details the steps and equipment utilized in...

CGMP Overview for Medical DevicesSEF-3001KG1

This course provides an overview of the Food and Drug Administration (FDA) principles, requirements, and expectations for compliance with Current Good Manufacturing Practices (CGMPs) related to medical device manufacture. This will include a review of FDA enforcement and the...

CGMP Overview for PharmaceuticalsSEF-3001KH1

An overview of Current Good Manufacturing Practices (CGMP) fundamentals used by pharmaceutical and biomanufacturing companies. CGMP exists to protect the health and safety of patients and consumers which supports the mission of the Food and Drug Administration (FDA). This co...

Cleaning and Disinfection for Aseptic Processing AreasSEF-159CL

Learn the role and importance of cleaning and disinfection in aseptic processing areas. Topics covered include regulatory requirements, cleanroom environments, basic microbiology, cleaning, disinfection and environmental monitoring. Class is a combination of lecture and hand...

Cleanroom Gowning CertificationSEF-79244

In the course's classroom component, students will learn about regulatory requirements and aseptic gowning techniques. Students will then learn aseptic behaviors related to gowning and how to gown in aseptic settings. After this formal training, students must successfully...

Cleanroom Gowning ConceptsSEF-3001JW1

Aseptic gowning is a system of donning apparel to prevent the contamination of aseptic processing areas. The Cleanroom Gowning course will establish a framework and requirements for proper sterile gloving and aseptic gowning procedures. E-learning tools and videos, and discu...

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Course

HPLC in Theory and Practice

Name

SEF-79624

Available Classes

Not currently offered.

Description Course Outline Requirements Intended Audience More Details

Description

High-performance liquid chromatography (HPLC) is a chemistry technique used to separate compounds of interest from a liquid mixture based on chemical and/or physical properties. When HPLC is coupled with a spectrophotometric detector, it can be used to quantify analytes to provide detailed information on the chemical composition of a sample. HPLC is commonly used in quality control or research laboratories in the biotechnology, chemical, agricultural, pharmaceutical, dietary supplement, and cosmetic industries. This course will focus on the basic applications of HPLC, including an introduction to separation science, detection methods, and the varieties of chromatography modes via HPLC. Additional topics include instrument orientation and operation, maintenance and troubleshooting, method development, sample preparation, and data analysis. Laboratory sessions will include hands-on experience in operation of an HPLC system.

Course Objectives

Be able to describe the importance of HPLC analyses in research and quality control
Understand various applications of HPLC
Develop a broad understanding of the design, operation and control of an HPLC system
Gain hands-on experience in basic HPLC method development, monitoring, maintenance, and troubleshooting

Outline of Instruction

Course introduction, pre-test
Introduction to Chromatography
Lab A - Lab visit, visual overview of HPLC equipment
Introduction to HPLC
Equipment and flow path
Basic Modes of operation
Produce standard calibration curve
Applications of HPLC
Lab B - Preparation of standards for auto-injection and setting up the auto-injector for overnight analysis
Lab C - Generating the calibration curve
Lab D - Preparation of standards for manual injection
Sample analysis and chromatogram interpretation
Statistical Analysis
Lab E - Manual injections and generation of calibration curve
Method development, maintenance, and troubleshooting
Course exam, review, Q&A

Contact Hours

CEU's

1.6

Industry Standard, State, or National Certification

CE to CU Articulation

Prerequisites

Participants should have taken at least one post-high school chemistry course that included a laboratory component, have a minimum of two years experience in a biotechnology-related or chemical industry, OR be a graduate of the BioWork program.

Text and Supplies Needed

Clinical Site/Special Facilities

- IBM “MT-M” computer
- Waters “Breeze” HPLC
- Waters “Breeze” 1500 Series HPLC pump
- Waters “Symmetry” reverse phase column
- 20 uL Injection syringe
- Waters 2487 dual wavelength absorbance detector
- Miscellaneous laboratory glassware and pipettes (multiple sizes)
- Solvents and chemical samples

Requirements for Successful Completion of this Course

Attendance 90% or above
Participation
Achieve a minimum score of 70% on the course exam.

Accreditation/Special Approval Requirements

Intended Audience

Specific Industry or Business Support Needs

Industry or Job Titles Related to training Outcomes for Employment

Related Courses

Chromatography in Theory and Practice - SEF-79614
Sample Preparation for HPLC Analysis - SEF-178CL

Course Contact Information

Bionetwork Manager

[email protected]

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Biotechnology

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Course details and registration information

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