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For those interested in earning a degree to work in the biotech industry, visit Wake Tech's Biotechnology Department.

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Course details and registration information

HPLC in Theory and Practice
Available Classes
Not currently offered.
Description Course Outline Requirements Intended Audience More Details
High-performance liquid chromatography (HPLC) is a chemistry technique used to separate compounds of interest from a liquid mixture based on chemical and/or physical properties. When HPLC is coupled with a spectrophotometric detector, it can be used to quantify analytes to provide detailed information on the chemical composition of a sample. HPLC is commonly used in quality control or research laboratories in the biotechnology, chemical, agricultural, pharmaceutical, dietary supplement, and cosmetic industries. This course will focus on the basic applications of HPLC, including an introduction to separation science, detection methods, and the varieties of chromatography modes via HPLC. Additional topics include instrument orientation and operation, maintenance and troubleshooting, method development, sample preparation, and data analysis. Laboratory sessions will include hands-on experience in operation of an HPLC system.
Course Objectives
  1. Be able to describe the importance of HPLC analyses in research and quality control
  2. Understand various applications of HPLC
  3. Develop a broad understanding of the design, operation and control of an HPLC system
  4. Gain hands-on experience in basic HPLC method development, monitoring, maintenance, and troubleshooting
Outline of Instruction
  1. Course introduction, pre-test
  2. Introduction to Chromatography
  3. Lab A - Lab visit, visual overview of HPLC equipment
  4. Introduction to HPLC
  5. Equipment and flow path
  6. Basic Modes of operation
  7. Produce standard calibration curve
  8. Applications of HPLC
  9. Lab B - Preparation of standards for auto-injection and setting up the auto-injector for overnight analysis
  10. Lab C - Generating the calibration curve
  11. Lab D - Preparation of standards for manual injection
  12. Sample analysis and chromatogram interpretation
  13. Statistical Analysis
  14. Lab E - Manual injections and generation of calibration curve
  15. Method development, maintenance, and troubleshooting
  16. Course exam, review, Q&A
Contact Hours
Industry Standard, State, or National Certification
CE to CU Articulation
Participants should have taken at least one post-high school chemistry course that included a laboratory component, have a minimum of two years experience in a biotechnology-related or chemical industry, OR be a graduate of the BioWork program.
Text and Supplies Needed
Clinical Site/Special Facilities
- IBM “MT-M” computer
- Waters “Breeze” HPLC
- Waters “Breeze” 1500 Series HPLC pump
- Waters “Symmetry” reverse phase column
- 20 uL Injection syringe
- Waters 2487 dual wavelength absorbance detector
- Miscellaneous laboratory glassware and pipettes (multiple sizes)
- Solvents and chemical samples
Requirements for Successful Completion of this Course
  1. Attendance 90% or above
  2. Participation
  3. Achieve a minimum score of 70% on the course exam.
Accreditation/Special Approval Requirements
Intended Audience
Specific Industry or Business Support Needs
Industry or Job Titles Related to training Outcomes for Employment
Related Courses
  • Chromatography in Theory and Practice - SEF-79614
  • Sample Preparation for HPLC Analysis - SEF-178CL
Course Contact Information
Bionetwork Manager