Wake Tech offers a variety of non-degree, short classes and certificate training programs in the biotechnology field, including hands-on training in biomanufacturing and biopharmaceutical operations in a simulated industrial, current good manufacturing practices (cGMP) environment.

Course Details & Registration Information

Sample Preparation for HPLC Analysis
Available Classes
    Section Location Begin Date End Date Instructor Price Seats Available/Total Seats
221815 BTEC BioNetwork Capstone Learning Center 04/30/2020 05/01/2020 G. Picard $232.00 12/12
Description Course Outline Requirements Intended Audience More Details
Gain hands-on (laboratory-based) experience in sample preparation and purification techniques for use in HPLC (High Performance Liquid Chromatographic) analysis of biological samples for trace levels of drug and pesticide residues. Students will perform standard preparation, reagent preparation, sample extractions, and typical purification procedures (solid-phase extraction). Participants will learn about the calibration of an HPLC system and follow an HPLC method developed for determination of trace levels of an organic contaminant in samples of milk.
Course Objectives
  1. Gain theoretical and practical hands-on experience with biological sample extraction and purification techniques.
  2. Learn basic analytical approaches and instrumentation used in trace organic analysis.
Outline of Instruction
  1. Introduction to bio-analytical (trace organic) analysis
  2. Sampling techniques
  3. Sample extraction techniques
  4. Sample extract purification including solid phase extraction and solvent partitioning techniques
  5. Basics of High Performance Liquid Chromatography (HPLC), top loading and analytical balances, pipettes, and volumetric flasks
  6. Labs: standard solution preparations, HPLC auto injection of calibration standards on an overnight run
  7. Calibration of an HPLC unit
  8. Importance of lab controls and lab fortified controls as quality control checks
  9. Method M 002 - Determination of a trace-level organic contaminant in milk
  10. Demonstration of Method M 002 to determine a trace-level organic contaminant in milk
  11. Labs: Perform Method M 002, injection of prepared samples, and calculation of the results
  12. Challenges posed by real world samples
Contact Hours
Industry Standard, State, or National Certification
CE to CU Articulation
Text and Supplies Needed
Clinical Site/Special Facilities
- HPLC equipped with a UV detector and both manual injector and a Waters Symmetry Reverse Phase Column
- Solid Phase Extraction cartridges and manifolds
- Analytical and top loading balance
- Vacuum pump
- High speed refrigerated centrifuge
- Reagents: methanol, acetonitrile, Milli-Q water, sodium chloride, potassium chloride, 5N hydrochloric acid, acetic acid, milk
- Assorted lab glassware and pipettes
- Imazaquin standard.
Requirements for Successful Completion of this Course
  1. Attendance 85% or above
  2. Participation
  3. Score of at least 70% on final exam.
Accreditation/Special Approval Requirements
Intended Audience
Specific Industry or Business Support Needs
Industry or Job Titles Related to training Outcomes for Employment
Related Courses
  • Chromatography in Theory and Practice - SEF-79614
  • HPLC in Theory and Practice - SEF-79624
Course Contact Information
Bionetwork Manager