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Course details and registration information

Sample Preparation for HPLC Analysis
Available Classes
Not currently offered.
Description Course Outline Requirements Intended Audience More Details
Students will execute basic analytical processes and use HPLC (High-Performance Liquid Chromatography) instrumentation to perform analysis of trace organic compounds. You will learn to analyze biological samples for trace levels of drug and pesticide residues using HPLC. Students will perform standard preparation, reagent preparation, and sample extractions for HPLC analysis. Participants also learn to follow a method developed to determine trace levels of an organic contaminant in biological samples.
Course Objectives
  1. Gain theoretical and practical hands-on experience with biological sample extraction and purification techniques.
  2. Learn basic analytical approaches and instrumentation used in trace organic analysis.
Outline of Instruction
  1. Introduction to bio-analytical (trace organic) analysis
  2. Sampling techniques
  3. Sample extraction techniques
  4. Sample extract purification including solid-phase extraction and solvent partitioning techniques
  5. Basics of High Performance Liquid Chromatography (HPLC), top loading and analytical balances, pipettes, and volumetric flasks
  6. Labs: standard solution preparations, HPLC auto injection of calibration standards on an overnight run
  7. Produce a standard calibration curve using model analyte
  8. Importance of lab controls and lab fortified controls as quality control checks
  9. Method M 002 - Determination of a trace-level organic contaminant in milk
  10. Demonstration of Method M 002 to determine a trace-level organic contaminant in milk
  11. Labs: Perform Method M 002, injection of prepared samples, and calculation of the results
  12. Challenges posed by real world samples
Contact Hours
Industry Standard, State, or National Certification
CE to CU Articulation
Text and Supplies Needed
Clinical Site/Special Facilities
- HPLC equipped with a UV detector and both manual injector and a Waters Symmetry Reverse Phase Column
- Solid Phase Extraction cartridges and manifolds
- Analytical and top loading balance
- Vacuum pump
- High speed refrigerated centrifuge
- Reagents: methanol, acetonitrile, Milli-Q water, sodium chloride, potassium chloride, 5N hydrochloric acid, acetic acid, milk
- Assorted lab glassware and pipettes
- Imazaquin standard.
Requirements for Successful Completion of this Course
  1. Attendance 85% or above
  2. Participation
  3. Score of at least 70% on final exam.
Accreditation/Special Approval Requirements
Intended Audience
Specific Industry or Business Support Needs
Industry or Job Titles Related to training Outcomes for Employment
Related Courses
  • Chromatography in Theory and Practice - SEF-79614
  • HPLC in Theory and Practice - SEF-79624
Course Contact Information
Bionetwork Manager