Course Offerings

High performance liquid chromatography (HPLC) is a chemistry technique used to separate chemical compounds-of-interest from other compounds in a liquid sample. When HPLC is coupled to a detection method, the compound- or compounds-of-interest can be quantified to provide detailed information on the chemical composition of a sample. HPLC is commonly used in quality control and research laboratories in the biotechnology, chemical, agricultural and pharmaceutical industries. This course focuses on basic applications of HPLC. Topics include an introduction to separation science and detection methods, types of HPLC, instrument orientation, operation, FDA criteria for bioanalytical methods and sample preparation, data analysis, and chromatogram interpretation. In addition, laboratory sessions provide hands-on experience using an Agilent HPLC system and Waters Empower 3 software.

Course Objectives

Explain the importance HPLC plays in research and quality control for pharmaceutical/biotechnological/ agricultural industry and on the various applications of HPLC

Describe the principles of chromatography, its uses and interpretation

Demonstrate a basic understanding of the design, operation and control of an Agilent HPLC system, including hands-on experience using Empower 3 software

Identify the FDA criteria for bioanalytical methods

Explain the importance of lab controls in QC

Outline of Instruction

Day 1: Introduction, pre-test, background on sample preparation, principles of chromatographic separation, basics of HPLC, IET HPLC video, importance of lab controls, FDA criteria for bio-analytical methods, assay of Walgreens Extra Strength Pain Reliever by HPLC: Method 005 (lab overview), instructor demonstration of Method 005, chromatogram interpretation

Day 2: Lab equipment demonstration videos, overview of Waters Empower 3 software, student hands-on solution preparation, student performance of Method 005, reporting results (lab), final Q&A, course wrap-up, course assessment and survey

Contact Hours

16

CEUs

No

Industry Standard, State or National Certification

Certification

None

Website

None

Certification Learning Outcomes/Requirements

CE or CU Articulation

No

Prerequisites

N/A

Learning Supplies Needed

HPLC machine, lab space, text and learning supplies will be provided.

Clinical Site/Special Facilities

Lab that has a HPLC machine

Requirements for Successful Completion

100% attendance

Accreditation/Special Approval Requirements

N/A

Intended Audience

Pharmaceutical, biomanufacturing, biotechnology positions for quality control, quality assurance, regulatory, engineering, laboratory, manufacturing or any other associate that needs a basic understanding of high-performance liquid chromatograph, especially quality control laboratory associates with the need to understand instrumentation related to HPLC

Specific Industry or Business Support Needs

Manufacturing Technician, Lab Technician, QC Analyst

Wake County Need for Industry Positions

N/A

Industry or Job Titles Related to Training Outcomes for Employment

Lab technician

Manufacturing technician

QC analyst

Related Courses

CTP-3001PD1

CTP-JI01

SEF-79634

Course Contact Information

Siobhan D'Antonio

[email protected]