BioNetwork Training

The BioNetwork Capstone Center provides affordable, high-quality, hands-on training in biotechnology, biomanufacturing, and biopharmaceutical/pharmaceutical operations in a simulated industrial (cGMP) environment.

The Capstone Center is situated in the Golden LEAF Biomanufacturing Training and Education Center (BTEC) at North Carolina State University Centennial Campus. It provides a training environment that mirrors a biomanufacturing plant facility with state-of-the-art classrooms, industrial-grade equipment laboratories, and a certified cleanroom suite.

Industry-recognized credential: BioNetwork Capstone Certificates

Complete in: 12 months or less

For non-credit student support services or to see if you qualify for financial assistance, contact the Career Pathways Program & Student Resources. 

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Course Details & Registration Information

Course
Preparation and Purification of Biological Samples for HPLC Analysis
Name
SEF-79824
Available Classes
Not currently offered.
Description Course Outline Requirements Intended Audience More Details
Description
Gain hands-on laboratory experience in sample preparation and purification techniques used in High Performance Liquid Chromatography (HPLC) analysis of biological samples for trace levels of drug and pesticide residues. Participants will prepare chromatography standards and reagents, perform sample extractions, complete purification procedures (liquid-liquid partitioning and solid-phase extraction), calibrate an HPLC system using both manual injection and automatic injection systems, and follow HPLC methods.
Course Objectives
  1. Understand the process for preparing a biological sample for HPLC analysis, including extraction and purification techniques
  2. Gain hands-on experience performing sample preparation and purification techniques
  3. Gain theoretical understanding and practical experience in the operation of HPLC instrumentation
Outline of Instruction
  1. Introduction to bio-analytical (trace organic) analysis
  2. Sampling techniques
  3. Sample extraction techniques
  4. Sample extract purification, including solid-phase extraction and solvent partitioning techniques
  5. The basics of High Performance Liquid Chromatography (HPLC)
  6. Instrumentation videos
  7. Lab – Standard solution preparation
    a. Preparation of a stock analytical standard solution
    b. Preparation of calibration and fortification standard solutions from the stock analytical standard solution using serial dilution technique
  8. Calibration of an HPLC unit
  9. Lab – Standard curves and reagent preparation
    a. Preparation of reagent solutions for Methods M 001, M 002, and M 003
    b. HPLC manual injection technique and generation of calibration data
    c. Generating HPLC standard curves from the manual injection standard calibration data
  10. The importance of lab controls and lab fortified controls as quality control checks
  11. Method M 001- Determination of a trace-level organic contaminant in water
    a. Overview and discussion
    a. Instructor demonstration of Method M 001
    b. Lab - student performance of Method M 001
  12. Method M 002 - Determination of a trace-level organic contaminant in milk
    a. Overview and discussion
    b. Instructor demonstration of Method M 002
    b. FDA validation criteria for a bio-analytical method
    c. Lab - Student performance of Method M 002
  13. Lab - HPLC mobile phase effects on chromatographic separations
    a. Mobile phase preparation
    b. Evaluation of mobile phase flow rate and composition on component retention times
  14. Lab - HPLC auto injection
    a. Prepare HPLC for auto-injection of calibration standards for an overnight run
    b. Generating HPLC standard curves using automatic injector data calibration data
  15. Challenges posed by real world samples
  16. Method M 003 - Determination of a trace-level organic contaminant in dry animal feed.
    a. Instructor demonstration of Method M 003
    b. Student performance of Method M 003
  17. Review and course exam
Contact Hours
32
CEU's
3.2
Industry Standard, State, or National Certification
No
CE to CU Articulation
No
Prerequisites
High school diploma, successful completion of BioWork program, or equivalent education.
Text and Supplies Needed
Clinical Site/Special Facilities
1. HPLC equipped with a UV detector and both manual and automatic injection systems
2. Waters Symmetry Reverse Phase Column
3. Solid Phase Extraction cartridges and manifolds
4. Analytical balance
5. Top loading balance
6. pH meter
7. Vacuum pump
8. High speed refrigerated centrifuge
9. Laboratory shaker
10. Reagents: methanol, acetonitrile, methylene chloride, Milli-Q water, sodium chloride, potassium chloride, 5N hydrochloric acid, and acetic acid
11. Milk and bunny food
12. Assorted lab glassware and pipettes
13. Imazaquin standard
Requirements for Successful Completion of this Course
  1. Attendance 90% or above
  2. Participation
  3. Achieve a minimum score of 70% on course exam.
Accreditation/Special Approval Requirements
Intended Audience
Specific Industry or Business Support Needs
Industry or Job Titles Related to training Outcomes for Employment
Related Courses
  • Sample Preparation for HPLC Analysis - SEF-178CL
  • HPLC in Theory and Practice - SEF-79624
  • Chromatography in Theory and Practice - SEF-79614
Course Contact Information
Willie Green-Aldridge
919-866-5145
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