Course Offerings

High-performance liquid chromatography (HPLC) is a chemistry technique used to separate chemical compounds-of-interest from other compounds in a liquid sample. When HPLC is coupled to a detection method, the compound- or compounds-of-interest can be quantified to provide detailed information on the chemical composition of a sample. HPLC is commonly used in quality control and research laboratories in the biotechnology, chemical, agricultural and pharmaceutical industries. This course focuses on basic applications of HPLC. Topics include an introduction to separation science and detection methods, types of HPLC, instrument orientation, operation and FDA criteria for bioanalytical methods, as well as sample preparation, data analysis and chromatogram interpretation. In addition, laboratory sessions provide hands-on experience using an Agilent HPLC system and Waters Empower 3 software.

Course Objectives

Explain the importance HPLC plays in research and quality control for pharmaceutical, biotechnology and agricultural industries and on the various applications of HPLC

Describe the principles of chromatography, its uses and interpretation

Demonstrate a basic understanding of the design, operation and control of an Agilent HPLC system, including hands-on experience using Empower 3 software

Identify the FDA criteria for bioanalytical methods

Explain the importance of lab controls in QC

Outline of Instruction

Overview: Learning objectives, polarity, introduction to chromatography, basics and real-world applications of HPLC, HPLC mobile phases and column, HPLC detectors, general lab safety, Agilent HPLC flow-path lab, standard and sample preparation for acetaminophen HPLC analysis, creating a mock run for acetaminophen HPLC analysis.

Review, integration, system suitability and quantitation; HPLC peak fronting, tailing and splitting; overview of Waters Empower 3 Software; integration, system suitability and quantitation of acetaminophen lab; mobile phase and column selection for multiple analytes; standard and sample preparation for acetaminophen, diphenhydramine HCl and dextromethorphan HBr lab; mobile phase solution prep and column set-up lab; and sample set-up for overnight run on HPLC lab.

Review; HPLC peak fronting, tailing and splitting; Waters Empower 3 Software advanced feature overview; integration, system suitability and quantitation of acetaminophen, diphenhydramine HCl and dextromethorphan HBr lab; HPLC troubleshooting lab; final Q&A; wrap-up, assessment and survey.

Contact Hours

24

CEUs

1

Industry Standard, State or National Certification

Certification

None

Website

None

Certification Learning Outcomes/Requirements

CE or CU Articulation

No

Prerequisites

Eligible participants should have taken at least one post-high school chemistry course that included a laboratory component. Prospective participants with a minimum of two years of experience in a biotechnology-related or chemical industry also qualify. BioWork students also meet the prerequisites.

Learning Supplies Needed

N/A

Clinical Site/Special Facilities

N/A

Requirements for Successful Completion

100% attendance

Accreditation/Special Approval Requirements

N/A

Intended Audience

Pharmaceutical, biomanufacturing, biotechnology positions for quality control, quality assurance, regulatory, engineering, laboratory, manufacturing or any other associate that needs a basic understanding of high-performance liquid chromatograph, especially quality control laboratory associates with the need to understand instrumentation related to HPLC.

Specific Industry or Business Support Needs

N/A

Wake County Need for Industry Positions

N/A

Industry or Job Titles Related to Training Outcomes for Employment

Related Courses

SEF-79634

CTP-JI01

Course Contact Information

BioNetwork Manager

[email protected]