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Upstream Processing Mammalian Suspension Cell Culture

Course ID: BTC-3300J6
Overview
Course Outline
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The initial phase in producing biopharmaceuticals is known as Upstream Processing. This process aims to optimize a cells growth and productivity while ensuring purity and quality. Mammalian cell culture is an important tool for research, clinical, and pharmaceutical applications. Through lecture and hands-on activities, participants will gain experience initiating and sub culturing a suspension cell line, observe for contamination, inoculate a small scale bioreactor, and perform an analysis to determine the growth rate of the cells.

Course Objectives

1. Learn about control strategies to use in upstream processing.
2. Develop an understanding of the phases of cell growth, cell counting, seed train, as well as aseptic technique when performing cell culture processes.
3. Gain a fundamental knowledge of suspension vs. adherent cell lines.
4. Gain hands-on experience initiating a mammalian cell line using Chinese Hamster Ovary (CHO) Suspension Cells.

Outline of instruction

Day 1: Lecture: Upstream Processing Overview
Lecture: Contamination Control affecting Processes
Lab: Cleaning the BSCs
Lab: Aseptic Technique
Lecture: Cell growth
Calculations Activity: C1V1=C2V2
Lecture: Reminder of the Importance of Contamination Control
Lecture: Production Introduction
Lab: CHO-S Suspension Cell Line Initiation
Lab: Subculture of Suspension Cells
Lab: Observing for contamination

Day 2: Lecture: Bioreactor Design
Lab: Bioreactor Identification
Lab: Inoculating the bioreactor
Lecture: Media composition & conditions
Lecture: Process Control
Lab: Check osmolality of sample
Lab: Metabolite analysis
Lecture: Upstream centrifugation considerations
Lecture: Upstream filtration considerations
Lecture: Review Q&A
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