Course Offerings

Microbiology Basics

Course ID: BTC-3300G6
Overview
Course Outline
Requirements
Intended Audience
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This course will explore basic, microbiology concepts that are necessary to know for topics appropriate for various positions within most life science organizations, including quality control associates. Participants will learn basic microbiological characteristics that are common in biomanufacturing facilities, as well as the sources of contamination related to personnel that work in these facilities. They will also learn the methods for monitoring, collecting, and testing of microorganisms that are objectionable in biomanufacturing environments, and the techniques to identify them so that these contaminants can be prevented from adversely impacting the product, as well as the methods and regulatory requirements for preventing contamination.

Course Objectives

Understand basic microbiology and properties of bacteria, fungi, and mycotoxins that can be harmful in biomanufacturing
Gain an understanding about the human impact on biomanufacturing operations.
Learn environmental monitoring and collection methods for effective microbial identification and management of contamination.
Understand the various analytical methods and identification techniques for identifying microorganisms so the sources of these contaminates may be prevented.
Learn the methods for disinfection, sterilization, and control of microbial contamination.
Gain an understanding of the importance for aseptic technique in preventing contamination of the product and test articles.

Outline of instruction

A. Introduction to Industrial Microbiology and Bacteria
a. Definition of microorganisms
b. Prokaryotes and Eukaryotes
c. General properties of bacteria
d. Aerobic vs anaerobic organisms
e. Medical and Industry Important Fungi (Mycoses, Mycotoxins)
B. Bacteria in humans
a. Spore forming bacteria
b. Bacterial Growth curve
C. Environmental Monitoring
a. Air contaminants
b. Surface contaminants
c. Personnel contaminants
d. Product contamination
e. Biofilms
f. Water collection Demo
D. Quality Control Laboratory Testing
a. Collection Techniques
b. Quadrant Streak for Isolation
c. Colony Morphology, Cell shape, and Gram stain
d. Detection vs. Quantification vs. Isolation
e. Analytical Profile Index
E. Identification Strategies
a. Preliminary Identification
b. Gram Stain
c. Historical Perspective on Bacterial Identification
d. Endotoxins
F. Contamination Control
a. Disinfection, Sterilization, Autoclaving
b. Aseptic Behaviors
G. Course Post Test
H. Course Evaluations