Biotechnology

Wake Tech offers a variety of non-degree, short classes and certificate training programs in the biotechnology field, including hands-on training in biomanufacturing and biopharmaceutical operations in a simulated industrial, current good manufacturing practices (cGMP) environment.


Course Details & Registration Information

Course
Basic Microbiological Laboratory Skills
Name
SEF-217CL
Available Classes
Not currently offered.
Description Course Outline Requirements Intended Audience More Details
Intended Audience
Microbiology Technicians, Quality Assurance Laboratory Technicians, Quality Control Technicians, Environmental Monitoring Technicians, Media Preparation Technicians, individuals seeking employment in FDA regulated microbiology laboratory
Description
This introductory course is for those interested in a career in a microbiology laboratory – or those new to the field who want to build basic skills. These skills are used in the manufacturing processes for a wide range of pharmaceutical, food, and dietary supplement products, all regulated by the FDA, USDA, and other agencies. The course covers techniques for safely handling, culturing, and examining microorganisms. Topics also include personal protective equipment (PPE), disinfection, aseptic technique, bacterial growth, isolation, microscope use, counting microbes in samples, and record keeping. The course will be held in a laboratory and will include short, informative lectures along with hands-on exercises using basic tools and equipment.
Course Objectives
  1. Be able to describe what microorganims are, their relative size, and those microorganism types commonly found and investigated in a FDA industrial setting.
  2. Be able to explain and demonstrate basic techniques of laboratory safety, disinfection, aseptic technique, bacterial growth, isolation, microscope use, and counting microbes in samples.
  3. Be able to execute minimum required record keeping for evaluating and tracking of laboratory samples and tests.
  4. Be able to demonstrate use of basic laboratory equipment and tools used in a microbiology laboratory.
Outline of Instruction
  1. Introduction
    A. Instructor and student introductions
    B. Questionnaires and preassessment
    C. Brief job description
    D. Goals for class, schedule, agenda, objectives
    E. Laboratory safety, BSL-1 and 2 practices
  2. Microbiology basics
    A. Domains of life
    B. Cell structure and size
  3. Safety
    A. General safety and location of emergency equipment
    B. Biosafety
    C. “Wet paint” theory in a laboratory
    D. Personal Protective Equipment (PPE)
    E. Hand washing and gloving
    F. Waste disposal
  4. Pipetting and serial dilutions
    A. Pipettes
    B. Micropipetting
    C. Multichannel pipetting
    D. Serial dilutions
  5. Disinfection
    A. Types of disinfectants
    B. Contact time, pH, temperature
    C. Process
  6. Record Keeping
    A. Time and temperature
    B. QC paperwork examples
    C. Labeling of supplies
  7. Aseptic Technique
    A. “Wet paint” theory applied to aseptic technique
    B. Minimizing Clutter
    C. Preventing aerosol formation
    D. Streaking plates
    E. Broths
    F. Slants
  8. Microscopy
    A. Parts of a brightfield light microscope
    B. Basic use of microscope to visualize pre-prepared slides
  9. Enumeration
    A. CFU theory
    B. Counting colonies on plates
    C. Turbidity measurements of previous day broth growth
  10. Review previous day plates for isolation and technique effectiveness
  11. Microbiology Common Equipment Overview
    A. Incubators
    B. Water baths
    C. Autoclaves
    D. Filters
  12. Conclusion, final discussion, assessment, evaluation
         
Contact Hours
16
CEU's
1.6
Industry Standard, State, or National Certification
No
CE to CU Articulation
No
Prerequisites
Text and Supplies Needed
Student handouts and laboratory supplies (BioNetwork provides)
Clinical Site/Special Facilities
BSL-2 Biosafety equipment required (BSC cabinet not needed)
Requirements for Successful Completion of this Course
  1. Attendance 90% or above
  2. Participation
Accreditation/Special Approval Requirements
Intended Audience
Microbiology Technicians, Quality Assurance Laboratory Technicians, Quality Control Technicians, Environmental Monitoring Technicians, Media Preparation Technicians, individuals seeking employment in FDA regulated microbiology laboratory
Specific Industry or Business Support Needs
Microbiology Technician, Quality Assurance Laboratory Technician, Quality Control Technician, Environmental Monitoring technician, Media Preparation Technician
Industry or Job Titles Related to training Outcomes for Employment
  • Microbiology Technician
  • Quality Assurance Laboratory Technician
  • Quality Control Technician
  • Environmental Monitoring technician
  • Media Preparation Technician
Related Courses
  • Basic Laboratory Skills - SEF-80004
  • Micropipetting Techniques for Precision & Accuracy - SEF-79654
  • Fundamentals of Microbiology - SEF-147CL
  • Advanced Microbiological Methods - SEF-201CL
Course Contact Information
Bionetwork Manager